Webseite von Dr. Rudolf Robelek
Webseite von Dr. Rudolf Robelek

Wissenschaftlicher Bereich

 

Profil / Tätigkeitsfelder

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​​Profil

 

Ausbildung /

Tätigkeiten

1999          

 

Diplom in Chemie (TU-München)

 

2002

 

Promotion im Bereich Proteinchemie (TU-München)

 

2003 - 2004   

 

Forschungsaufenthalt an der NUS und dem IMRE in Singapur

 

2005 - 2007

 

Postdoktorat am Max-Planck-Institut für Polymerforschung Mainz

 

2008 - 2014              

 

Lehr- und Forschungstätigkeit an der Universität Regensburg

 

2014

 

Habilitation im Fach Anayltische Chemie / Biosensorik an der Universität Regensburg

 

Seit 2014 Hochschuldozent und Leiter der Verwaltung am Institut für Anorganische und Analytische Chemie der JGU Mainz

 

 

 

 

 

Wissenschaftliche Veröffentlichungen

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Originalarbeiten

 

 

Wurster, E.C.; Liebl, R.; Michealis, S.; Robelek, R.; Wastl, D.S.; Giessibl, F.J.; Goepferich, A.; Breunig, M. (2015) Oligolayer-Coated Nanoparticles: Impact of Surface Topography at the Nanobio Interface. ACS Applied Materilas & Interfaces, 7 (15), 7891-7900.

 

Michaelis, S.; Wegener, J.; Robelek R. (2013) Label-Free Monitoring of Cell-Based Assays: Combining Impedance Analysis with SPR for Multiparametric Cell Profiling. Biosensors & Bioelectronics, 49, 63-70.

 

Vala, M.; Robelek,  R.; Bocková, M.; Wegener, J.; Homola, J. (2013) Real-time label-free monitoring of the cellular response to osmotic stress using conventional and long-range surface plasmons. Biosensors & Biolectronics, 40 (1), 417-421.

 

Robelek, R. (2012)  Nicht-invasive, label-freie Analytik lebender, tierischer Zellen mittels Oberflächen Plasmon Resonanz Sensoren. GIT, 56 (4), 259-263.

 

Baumgarten, S.; Robelek, R. (2011) Surface Plasmon Resonance (SPR) Sensors for the rapid, sensitive detection of the cellular response to osmotic stress. Sensors and Actuators B, 156 (2), 798-804.

 

Robelek, R.; Wegener, J. (2010) Label-free and Time-Resolved Measurements of Cell Volume Changes by Surface Plasmon Resonance (SPR) Spectroscopy. Biosensors & Bioelectronics, 25 (5), 1221-1224.

 

Leutenegger, M.; Lasser, T.; Sinner, E.K.; Robelek, R. (2008)  Imaging of G protein-coupled receptors in solid-supported planar lipid membranes. Biointerphases, 3 (2), FA136-FA145.

 

Marki, I.; Leutenegger, M.; Geissbühler, M.; Robelek, R.; Sinner, E.K.; Lasser, T. (2008) Imaging of G protein-coupled receptors in solid-supported planar membranes at the single molecule level. Single Molecule Spectroscopy and Imaging, 6862 (34), 68620X.

 

Robelek R.; Leutenegger M.; Wiltschi B.; Lemker E.S.; Oesterhelt D.; Lasser T.; Sinner E.K. (2007) In vitro synthesis of GPCRs in a solid supported planar lipid membrane system. Biophys. J., 38A Suppl. S.

 

Robelek, R.; Lemker, E.; Wiltschi, B.; Kirste, V.; Naumann, R.; Oesterhelt, D.; Sinner, EK. (2007)   Incorporation of in vitro Synthesized GPCRs into a Tethered Artificial Lipid Membran System. Angew. Chem. Intl. Ed., 46; 605-608.

 

Robelek, R.; Stefani, F., Knoll, W. (2006) Oligonucleotide Hybridization Monitored by Surface Plasmon Enhanced Fluorescence Spectroscopy with Bio-Conjugated Core/Shell Quantum Dots. Influence of Luminescence Blinking. Phys. Stat. Sol. (a). 203(14); 3468-3475.

 

Knoll, W.; Zong, X.; Stefani, F.; Robelek, R.; Niu, L.; Rochholz, H.; Shumaker-Parry, J; Kreiter, M. (2006) Optics with Nano-Sized Structures Made from Semiconductors and (Noble) Metals. JNOPM, 15(3), 355-367.

 

Su, X.; Wu, Y-J.; Robelek, R., Knoll, W. (2005)  Surface Plasmon Resonance Spectroscopy and Quartz Crystal Microbalance Study of Streptavidin Film Structure Effects on Biotinylated DNA Assembly and Target DNA Hybridization. Langmuir, 21(1); 348-53.

 

Su, X.; Wu, Y-J.; Robelek, R.; Knoll, W. (2005) Surface Plasmon Resonance Spectroscopy and Quartz Crystal Microbalance Study of MutS Binding with Single Thymine-Guanine Mismatched DNA. Front. Biosci., 10; 268-274.

 

Bonin, I.; Robelek, R.; Benecke, H.; Urlaub, H.; Bacher, A.; Richter, G.; Wahl, M.C. (2004) Crystal Structures of NusB from Thermotoga maritima and Implications for RNA Binding. Biochem J., 383(3); 419-28.

 

Robelek, R.; Niu, L.; Schmid, E.L.; Knoll, W. (2004) Multiplexed Hybridization Detection of Quantum Dot Conjugated DNA Sequences using Surface Plasmon Enhanced Fluorescence Microscopy and Spectrometry. Anal. Chem., 76(20); 6160-6165.

 

Knoll, W.; Tamada, K.; Yu, F.; Robelek, R.; Niu, L.; Yao, D.; Han, M.; Zhong, X.; Schmid, E.L. (2004) Supramolecular interfacial architecture for biosensing. Polymeric Materials Science and Engineering, 90, 687- 688.

 

Knoll, W.; Han, M-Y.; Li, X.; Fernandez-Lopez, J-L.; Manna, A.; Müllen, K.; Nakamura, F.; Niu, L.; Robelek, R.; Schmid, E.L.; Tamada, K.; Zhong, X. (2004) Nanoscopic Building Blocks from Polymers, Metals, and Semiconductors for Hybrid Architectures. JNOPM., 13(2), 229ff.

 

Su, X.; Robelek, R.; Wu, Y.; Wang, G.; Knoll W. (2004) Detection of Point Mutation and Insertion Mutations in DNA using Quartz Crystal Microbalance and MutS, a Mismatch Binding Protein. Anal. Chem., 76(2), 489-494.

 

 

Robelek, R.; Mühlberger, R.; Eisenreich, W.; Ettenhuber, C.; Sinner, E.-K.; Kessler, H.; Bacher, A. and Richter, G. (2003) RNA DNA discrimination by the antitermination protein NusB. J. Mol. Biol., 327, 973-83.

 

Lüttgen, H.; Robelek, R.; Mühlberger, R.; Diercks, T.; Schuster, S. C.; Köhler, P., Kessler, H.; Bacher, A.; Richter, G. (2002) Transcriptional regulation by antitermination. Interaction of RNA with NusB protein and NusB/NusE protein complex of Escherichia coli. J. Mol. Biol., 316, 873-83.  

 

 

 

Review-Artikel und Buchbeiträge

 

Michaelis, S.; Robelek, R.; ·Wegener, J. (2011) Studying Cell-Surface Interactions In Vitro: A Survey about Experimental Approaches and Techniques. Tissue Engineering III, Advances in Biochemical Engineering / Biotechnology., ISSN 1616-8542.

 

Robelek, R. (2009) Surface Plasmon Resonance Sensors in Cell Biology: Basics and Applications. Bioanal. Rev., 1 (1), 57-72.

 

Knoll, W.; Kasry, A.; Liu. J.; Neumann, T.; Niu, L.; Park, H.; Paulsen, H.; Robelek, R.; Yu, F. (2008)  Surface Plasmon Fluorescence Techniques for Bioaffnity Studies. Handbook of Surface Plasmon Resonance (Chapter 9); Royal Society of Chemistry; ISBN: 978-0854042678.

 

 

 

Akademische Arbeiten

 

Robelek, R. (2014) Surface Plasmon Resonance Based Biosensors: From Bimolecular Interaction Towards Whole Cell Anaylsis. Habilitationsschrift, Universität Regensburg.

 

Robelek, R. (2002) Studies of transcriptional antitermination in Escherichia coli. Doktorarbeit, Technische Universität München.

 

Robelek, R. (1999) Untersuchungen zur Antitermination in Escherichia coli. Diplomarbeit, Technische Universität München.

 

 

 

Patente

 

Robelek, R.; Lempker, E.; Wiltschi; Sinner, E.K., (2006) Cell-free in vitro transcription and translation of membrane proteins into tethered lipid layers. European Patent, PCT/EP 06/008318.

 

Su, X.; Robelek, R.; Knoll, W.; O’Shea, S. (2005) Piezoelectric quartz crystal microbalance and method for high-throughput detection of genetic polymorphisms associated with diseases. US Patent Publication, Patent# US 2005164236.

 

Su, X.; Robelek, R.; Knoll, W.; O’Shea, S. (2005) Device and Method of Detecting Mutations and Polymorphisms in DNA. SG Patent Publication, Patent# SG 110153A.

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